RORgt Antibody From eBioscience Gives Clear Intra-Nuclear Staining

December 22, 2023

BlueskyReddit
Medicine
Weill Cornell Medicine
Research Scientist

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Company:

eBioscience

Product Name:

ROR gamma (t) Monoclonal Antibody, PE-eFluo-610

Catalog Number:

61-6981-82

Clone Number:

B2D

When used for flow cytometry on intestinal cells, there will be some background.But, the RORgt cell population is clear.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

Membrane staining in PBS

Blocking Agent

Fc Block

Secondary Incubation

Intranuclear staining eBioscience FOXP3 Fix/Perm buffer

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

Mouse intestinal innate lymphoid cells stained with this antibody show a clear population of RORgt+ cells that correspond to group 3 innate lymphoid cells (ILC3s). The staining is good. The shift is limited but good and normal for this type of marker. Titration must be performed to improve the staining at the maximum

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good and specific staining.

The Bad

Require antibody titration to improve the staining at the best.

The Bottom Line

This is a good flow cytometry antibody. As it required intracellular staining and the RORgt marker has a limited shift, I strongly recommend performing titration for this antibody.

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