Reliable Beads for Compensation Using Amine Dyes

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

ArC™ Amine Reactive Compensation Bead Kit

Catalog Number:

A10346

We differentiate dendritic cells from CD14+ PBMCs with the goal of assessing dendritic cell maturation/activation by flow cytometry after different treatments.

Experimental Design and Results Summary

Application

Flow cytometry compensation

Starting Material

Tips

Stain in an eppendorf or flow tube for maximal bead recovery.

Results Summary

This product helps us stain beads with our amine-reactive fixable live/dead stains, which then allows for easy flow cytometry compensation when evaluating live/dead populations. We've been able to assess viability, as well as use this for live/dead gates in DC maturation studies.

DOI or PMID #

N/A

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Summary

The Good

Easy to use and reliably stains.

The Bad

Requires addition of negative beads after staining.

The Bottom Line

Strongly recommend these beads as a compensation control for amine dyes.

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