Excellent Antibody for CD3 From BD Biosciences

December 22, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Pharmingen™ PE Hamster Anti-Mouse CD3e

Catalog Number:

553064

Clone Number:

145-2C11

Info:Supplier Page 

Browse Similar Products: Anti-CD3E Antibody Products

This antibody gives good separation of CD3-positive cells in mouse intestines. The signal is bright and specific.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Single cell suspensions from mouse intestines stained with this antibody show a clear population of CD3 positive cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

N/A.

The Bottom Line

The staining for CD3 positive cells with this antibody is strong and specific. It makes gating T cells very easy.

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