Great DNA Stain for qPCR From Invitrogen

November 03, 2023

BlueskyReddit
University of California San Francisco
Biomedical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

SYBR-Green Nucleic Acid Stain

Catalog Number:

S7563

Our lab is interested in using cutting-edge technologies to better understand the mechanisms responsible for immune self-tolerance and self-antigen production/presentation during both central and peripheral tolerance.

Experimental Design and Results Summary

Application

qPCR

Starting Material

Mouse epithelial cell genomic DNA

Tips

Make sure the qPCR instrument is properly and recently calibrated for use of SYBR reagents.

Results Summary

After optimization, this DNA stain works extremely well as a sensitive readout of amplification during qPCR.

DOI or PMID #

N/A

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Summary

The Good

Sensitive and cheap.

The Bad

Does require some optimization for specific applications.

The Bottom Line

A great general purpose DNA stain for qPCR after the protocol has been properly optimized for the specific application/amplification being performed.

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