Great Anti-Human CD45 Antibody From BioLegend is Easy to Work With

October 16, 2023

BlueskyReddit
Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-human CD45 Antibody

Catalog Number:

304042

Clone Number:

We are a cancer immunotherapy group, using cell-based approaches. Ultimately our products are tested in animal models, so good markers are needed to separate the human from the mice cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Lymphocytes

Primary Incubation

1:100, 15min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/a

Detection

BD LSR II

Results Summary

We routinely use this antibody in our in vivo experiments in combination with a mouse a-CD45. The combination of these 2 antibodies give a clear separation of the the human/mouse cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use and works wells.

The Bad

None.

The Bottom Line

A good antibody that gives a decent signal and requires low amounts of it for the staining. BV605 might not be the brightest fluorochrome, but other options are available for the same clone if stronger signal is required.

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