Abcam
Anti-Lamin B1 antibody
ab65986
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We aimed to detect lamin B1 using rat brain samples, through IHC-IF. Overall, this antibody works fine.
Immunohistochemistry
Rat brain
1 µg/ml
Normal Goat Serum diluted in 0.1% BSA
AlexaFluor 555 goat anti-rabbit
None
Confocal - fluorescence
Antigen retrieval: heat-mediated with 10 mM TRIS-EDTA buffer (pH 9)The antibody detects well Lamin B1 in the brain of rat samples. Not all cells express Lamin B1 at the same levels (e.g., neurons seem to have more than endothelial cells).
N/A
Easy optimization.
Signal intensity is low in some cells.
Great, we use it for ICC and IHC-IF.