Miltenyi Biotech
CytoStim™, human
130-092-172
Info:Supplier Page
I used cytoStim reagent as a positive control to restimulate CD4+ and CD8+ T-cells present in PBMCs. After stimulation for 4-8h PBMCs were stained with fluorochrome antibodies to check the increase in expression of surface markers.
Stimulation of human T-cells
PBMCs
If possible use freshly isolated PBMCs
5-10% increase in the expression of activation surface markers on T-cells were observed compared to non-stimulated PBMCs.
N/A
Easy protocol and non-toxic reagent.
Bit expensive.
Works great as a positive control to standardize your protocol for test samples.