Invitrogen
F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor™ 488
A11070
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Goat anti-rabbit secondary antibody tagged with Alexa Fluor 488 is tested for immunofluorescence. Used at a concentration of 2.5 ug/ml for an hour at room temperature. Very specific and excellent signal. This is an extremely specific pre-absorbed antibody with no autofluorescence or non-specific signal.
Immunofluorescence
Monocyte derived macorphages
PBS with 1% BSA + 0.3% Triton X-100
PBS with 5% normal goat serum + 0.3% Triton X-100
2.5ug/ml
1 hr at room temperature
Immunofluorescence detection by confocal microscopy
Clear signal with no autofluorescence and/or background. It is a perfect fluorescence-tagged antibody for immunofluorescence studies.
N/A
Works great.
None.
Highly recommended for IF studies.