Puromycin From Thermo Fisher is Ideal for Antibiotic Selection

October 20, 2023

BlueskyReddit
UNC Chapel Hill

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Fisher

Product Name:

Puromycin, 10 mg/ml in distilled water, sterile-filtered, Thermo Scientific Chemicals

Catalog Number:

J67236.8EQ

Puromycin (puromycine) is an aminoglycoside antibiotic originating from Streptomyces alboniger. It acts as an aminoacyl tRNA analog and prematurely terminates peptide elongation. It has effects on both prokaryotes and eukaryotes.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Cultured mammalian cells

Tips

I use the dilution as recommended

Results Summary

I use it for antibiotic selection and it works great. I have been using it for a long time now. It works well.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Works well with all cell lines.

The Bad

Can't think of anything bad.

The Bottom Line

I use 1:1000 dilution and it has worked well so far for me. I have used it with multiple lentiviral transfections, and with different cell lines. I also add it routinely to my stable cell lines.

Join the discussion