Very Good CD64 Antibody From BioLegend

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD64 (FcγRI) Antibody

Catalog Number:

139323

Clone Number:

X54-5/7.1

Great CD64 antibody to use when staining intestine immune cells. Clear positive population, although the signal could be a bit brighter.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse immune cells from intestines stained with this antibody show a clear positive population, although staining could be a bit brighter.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining for macrophages.

The Bad

A little bit dim.

The Bottom Line

Although the staining is a little bit on the dim side, the staining is specific and shows a clear positive population.

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