Decent CD19 Antibody From BD Biosciences

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BD Horizon™ BV711 Mouse Anti-Human CD19

Catalog Number:

563038

Clone Number:

SJ25C1

Info: Supplier Page 

Browse Similar Products: Anti-CD19 Antibody Products

When used for flow cytometry on intestinal cells, there will be some background, but the B cell population is clear.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4 degrees C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Mouse intestinal immune cells stained with this antibody show a clear population of SSCA low CD19+ cells. The staining is a little dim compared to other antibodies.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear CD19 positive population.

The Bad

Some background is present.

The Bottom Line

The fluorochrome of this antibody will lead to some compensation issues, but overall staining is decent.

Share your experience with other scientists. Write a Review! »

Join the discussion