BioLegend
PE/Cyanine7 anti-human CD4 Antibody
357410
A161A1
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I used this antibody to determine what percentage of my T cell product was CD4+. I was able to quantify the subpopulation with no issues.
Flow cytometry
Primary human T cells
1:100 dilution, 30 minute incubation at 4C in the dark
None
This antibody allowed me to identify the percentage of CD4+ T cells in my expanded T cell product. This antibody produced good signal and the analyzed population was clearly distinct from my CD8+ population.
N/A
Good signal and no optimization is required.
None.
I would repurchase this antibody without question.