Cytiva
Trypsin 0.05% &protease solution with porcine trypsin, HBSS, EDTA; without calcium, magnesium
SH30236.02
Info:Supplier Page
We use this on adherent tumor cells to dislodge them from TC-treated flasks. After removing the media, we put enough to cover the bottom and waited 5 minutes before "power-washing" with a pipet tip. Cells come off very quickly in one wash without scraping or tapping. Trypsin can be more harsh on other cells and change their membrane proteins, so we do not use it on our T cells or DCs.
Dislodging tumor cells
Adherent tumor cells
I use my pipet tip to "power wash" the bottom of the flask after allowing trypsin to sit for 5 minutes.
We use this on adherent tumor cells to dislodge them from TC treated flasks. After removing media, we put enough to cover bottom and wait 5 minutes before "power-washing" with pipet tip. Cells come off very easily in one wash without need for scraping or tapping. Trypsin can be more harsh on other cells and change their membrane proteins, so we do not use it on our T cells or DCs.
N/A
Easy and quick.
Can be harsh on some types of cells.
Great for stubborn adherent tumor cells.