Ideal CK18 Antibody From Abcam

Pathology
Sao Paulo State University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Cytokeratin 18 antibody

Catalog Number:

ab668

Clone Number:

N/A

This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human amnion epithelial cells

Primary Incubation

1:500 dilution, overnight at 4 degrees

Blocking Agent

3% Bovine Serum Albumin (BSA)

Secondary Incubation

1:400 Mouse antibody, 1 hours, RT

Tertiary Incubation

N/A

Detection

Zeiss 880 confocal microscope

Results Summary

The CK18 antibody used for immunofluorescence purposes on amnion epithelial cells gave satisfying results. Minimum to no background was observed.

DOI or PMID #

10.1530/REP-20-0283

Additional Notes

N/A

Related Categories

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Summary

The Good

Excellent quality.

The Bad

N/A

The Bottom Line

Very suitable and quality antibody for IFF.

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