Brilliant Actin Antibody For Western Blot From Millipore

September 11, 2023

BlueskyReddit
Pathology
Sao Paulo State University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Antiβ-actina monoclonal

Catalog Number:

A5441

Clone Number:

N/A

This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB)

Experimental Design and Results Summary

Applications

Western blot

Sample

Human amnion epithelial cells

Primary Incubation

1:10.000 dilution, overnight at 4 degrees

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:15000, 2 hours, RT

Tertiary Incubation

N/A

Detection

FACSCalibur (Becton Dickinson), software CellQuest version 4

Results Summary

The action antibody gave satisfying results in WB analysis. The band is clear and the run does not have a lot of background.

DOI or PMID #

10.1530/REP-20-0283

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good quality and low background.

The Bad

N/A

The Bottom Line

Very good signal, appropriate results for this marker.

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