Great Antibody for TAB1 (WB) From R&D Systems

September 08, 2023

Pathology
Sao Paulo State University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

TAB1 antibody

Catalog Number:

AF3578

Clone Number:

N/A

This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB)

Experimental Design and Results Summary

Applications

Western blot

Sample

Human amnion epithelial cells

Primary Incubation

1 hour at 4°C in blocking reagent (1:1000 dilution)

Blocking Agent

5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)

Secondary Incubation

1:5000, 2 hours, RT

Tertiary Incubation

N/A

Detection

FACSCalibur (Becton Dickinson), software CellQuest version 4

Results Summary

The results look good using this dilution. The band is clear and the run has a minor background without compromising the result.

DOI or PMID #

10.1530/REP-20-0283

Additional Notes

N/A

Image Gallery

Summary

The Good

Good quality and low background.

The Bad

N/A

The Bottom Line

Good signal, appropriate results for this marker.

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