Abcam
Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker
ab98952
N/A
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This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB). It was published in Reproduction in 2020 (Silva et al., Inflammation, but not infection, induces EMT in human amnion epithelial cells. Reproduction. 2020 Oct;160(4):627-638).
Western blot
Human amnion epithelial cells
1 hour at 4°C in blocking reagent (1:600 dilution)
5% nonfat milk in 1× Tris-buffered saline-Tween 20 (TBS-T)
1:10000, 2 hours, RT
FACSCalibur (Becton Dickinson), software CellQuest version 4
The results looks good using this dilution. The band is clear and the run does not have a lot of background.
10.1530/REP-20-0283
Good quality and low background.
The dilution used was different from what the manufacturer suggested.
Good signal, appropriate results for this marker.