Effective Kit From Promega to Measure Extracellular ATP

George Washington University
Immunology
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

RealTime-Glo™ Extracellular ATP Assay

Catalog Number:

GA5010

I used this assay kit to measure ATP in cell culture supernatant following treatment. This kit allowed me to quantify the amount of ATP released by dying cells and thereby evaluate the viability of my cells following treatment.

Experimental Design and Results Summary

Application

Evaluating extracellular ATP in measuring viability and cytotoxicity.

Starting Material

Cells

Tips

None.

Results Summary

This kit allowed for a sensitive and rapid quantification of ATP released by cells as a measurement of cell viability. From this kit, I was able to demonstrate dose-dependent cytotoxic effects of my treatment.

DOI or PMID #

N/A

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Summary

The Good

Can be completed in under 30 minutes.

The Bad

Highly sensitive so replicates are important.

The Bottom Line

This kit is time and cost-effective and provides an a clear evaluation of extracellular ATP.

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