BCA Protein Assay Kit From ThermoFisher is a Reliable Method for Measuring Protein Concentration

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific™

Product Name:

Pierce™ BCA Protein Assay Kits

Catalog Number:

23225

Clone Number:

N/A

To use an accurate amount of protein for my experiments, I use the BCA Protein Assay as my preferred quantification method. This kit is extremely efficient and can be done within an hour and easily read out on a spectrophotometer.

Experimental Design and Results Summary

Applications

Protein quantification

Sample

Protein

Primary Incubation

30 minutes at 37 degrees C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Spectrophotometer

Results Summary

This kit enables an efficient method for quantifying the amount of protein I have in my sample. It is a robust and reliable quantification that is not sensitive to detergents like other protein assay kits are. For most applications, I would default to using the BCA Protein Assay kit.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Standards come pre-diluted, assay can be completed in an hour.

The Bad

None.

The Bottom Line

This kit is a staple in our lab to quantify protein as it is efficient and reproducible.

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