BioLegend's BV421 Anti-Mouse CD4 Ab Works Perfectly

September 11, 2023

BlueskyReddit
Department of Fundamental Oncology
University of Lausanne
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

BV421 anti-mouse CD4 Antibody

Catalog Number:

100543

Clone Number:

RM4-5

To titrate the antibody, 5 x 10^5 splenocytes from C57BL/6 mice were stained with PerCP anti-CD8a Ab and BV421 anti-CD4 Ab in the presence of Zombie NIR in 50 µL PBS at 4C for 30 min.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 5 x 10^5 splenocytes from C57BL/6 mouse were stained with: PerCP anti-CD8a Ab and BV421 anti-CD4 Ab in the presence of Zombie NIR in 50 μL PBS at 4C for 30 min.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great resolution.

The Bad

N/A

The Bottom Line

According to the titration, 1:50 dilution has the best SI.

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