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Alexa Fluor 700 anti-mouse CD8a Antibody
100730
53-6.7
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To titrate the antibody, 5 x 10^5 splenocytes from C57BL/6 mice were stained with: PerCP-Cy5.5 anti-CD4 Ab, AF700 anti-CD8a Ab in the presence of Zombie NIR in 50 µL PBS at 4C for 30 min.
Flow cytometry
Splenocytes from C57BL/6 mouse
See description
N/A
BD LSRFortessa
To titrate the antibody, 5 x 10^5 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD4 Ab, AF700 anti-CD8a Ab in the presence of Zombie NIR in 50 μL PBS at 4C for 30 min.
Great resolution.
According to the titration, 1:100 dilution has the best SI.