Good Quality IFN gamma ELISA Based Kit From Invitrogen

September 07, 2023

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

IFN gamma Human ELISA Kit

Catalog Number:

EHIFNG

Clone Number:

N/A

This IFN-γ ELISA kit has excellent sensitivity and range of detection. I've never had any issues with this kit and have successfully used this kit several times with reproducible results.

Experimental Design and Results Summary

Applications

ELISA

Sample

Cell culture supernatant

Primary Incubation

2 hours

Blocking Agent

None

Secondary Incubation

30 minutes

Tertiary Incubation

30 minutes

Detection

Absorbance by spectrophotometer

Results Summary

This kit helped to quantify the amount of IFN-γ release by my T cells. The samples in my plate were all within the standard included in the kit and I was able to measure the concentration of soluble protein in my T cell supernatant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Pre-coated, straight foward instructions, and is completed all within a day.

The Bad

Pricey.

The Bottom Line

While expensive, this kit is reliable and is simple to use. Because it's pre-coated, the kit saves a great deal of time so that you can complete your assay within hours.

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