A Necessity in Your Flow Cytometry Panel From Invitrogen

September 07, 2023

BlueskyReddit
Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

UltraComp eBeads™ Compensation Beads

Catalog Number:

01-2222-41

Clone Number:

N/A

Info: Supplier Page 

I always use UltraComp beads for my single stain controls in my flow cytometry panel. They are reliable, consistent, and easy to use. It's especially great that both the positive and negative beads are included in this mix to simplify your workflow

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Single stain antibody control

Primary Incubation

30 minutes at 4 degrees C in the dark

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

UltraComp beads help me achieve compensation of fluorochrome spillover. These beads allow me to separate positive and negative signals for every single marker and appropriately adjust voltages and set gating parameters so that I can achieve the highest quality readout for my samples.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Includes both positive and negative beads and work better on difficult fluorophores.

The Bad

None.

The Bottom Line

UltraComp Compensation Beads are essential for compensation controls. Of the beads that I have tested for my experiments, UltraComp is one of the better beads on the market. It's extremely simple and efficient to use and I highly recommend it to flow cytometry users.

Share your experience with other scientists. Write a Review! »

Join the discussion