Good RANKL Antibody From R&D Systems

September 08, 2023

BlueskyReddit
Surgery
University of Texas Southwestern Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

RANKL antibody

Catalog Number:

AF462

Clone Number:

N/A

I used this to detect RANKL expression in mouse mammary glands with or without knockout of a gene of interest. We observed that RANKL expression decreased in the KO mouse mammary tissues. The antibody showed a very clear band at around 35-40kDa.

Experimental Design and Results Summary

Applications

Western blot

Sample

Mouse mammary gland

Primary Incubation

1:1000, 4 degrees, O/N

Blocking Agent

5% non-fat milk

Secondary Incubation

1:20000, 1h at RT

Tertiary Incubation

None

Detection

Amersham ECL and picture taken using Chemidoc

Results Summary

This antibody detects a clear band at the expected size with minimal background noise. It works well for cell or tissue lysates from mouse origin.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Clear band and no background.

The Bad

N/A

The Bottom Line

Reliable RANKL antibody.

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