Abcam
Recombinant Anti-HIF-1 alpha antibody
ab51608
N/A
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I used this antibody to check HIF1A expression in colorectal cancer cell lines, Colo205 and HT29. I used 40ug of proteins. Running was done at 80-100v for 2h and wet transfer was done at 100v for 1.5h. Blocked using 5% non-fat milk and primary dilution at 1:1000 at 4 degrees overnight. The antibody worked well and gave a clear band at around 130KDa.
Western blot
Human colorectal cancer cell line Colo205 and HT29
1:1000, 4 degrees, O/N
5% non-fat milk
1:20000, 1h at RT
None
Amersham ECL and picture taken using Chemidoc
I used this antibody to check HIF1A expression in colorectal cancer cell lines, Colo205 and HT29. I used 40 ug of proteins. Running was done at 80-100v for 2h and wet transfer was done at 100v for 1.5h. Blocked using 5% non-fat milk and primary dilution at 1:1000 at 4 degree overnight. The antibody worked well and gave a clear band at around 130KDa.
NA
Clear band, and no background.
Cost is higher.
Great signal, expensive but worth it.