Excellent Antibody for Immunofluorescence

August 07, 2023

BlueskyReddit
Virginia Commonwealth University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

HMGB1 antibody

Catalog Number:

3935

Clone Number:

N/A

I stained human macrophages with this rabbit anti-HMGB1 antibody to identify the HMGB1 location in the cell. It works great!

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human macrophages

Primary Incubation

1:100, 4°C for overnight

Blocking Agent

Normal goat serum (5%) as blocking agent for 1h

Secondary Incubation

Anti-rabbit conjugated with alexa fluor 488, 2.5ug/ml for 1.5h at RT

Tertiary Incubation

None

Detection

Immunofluorescence detection by confocal microscopy

Results Summary

Highly recommended.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Ready to use and reliable.

The Bad

None.

The Bottom Line

I stained human macrophages with this rabbit anti-HMGB1 antibody to identify the HMGB1 location in the cell. It works great!

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