Good Antibody From ThermoFisher, but Has Some background

August 04, 2023

BlueskyReddit
Wayne State University

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Company:

ThermoFisher Scientific

Product Name:

IgD Monoclonal Antibody (IA6-2), FITC, eBioscience™

Catalog Number:

11-9868-42

Clone Number:

IA6-2

Great antibody for IF, but there is a little background in the image. Also works for flow cytometry.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human tonsil

Primary Incubation

1:100 overnight at 4°C

Blocking Agent

5% BSA in PBST

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZEISS LSM880

Results Summary

Human tonsil sections stained with this antibody show strong cytoplasmic IgD staining. There is some background, but specific signal is stronger.

DOI or PMID #

N/A

Additional Notes

Also works for flow cytometry.

Related Categories

Image Gallery

Summary

The Good

Good for staining human IgD.

The Bad

Some background is present.

The Bottom Line

Good antibody for staining IgD in human samples for either flow cytometry or imaging.

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