Strong B220 Staining With Antibody From BioLegend

August 04, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cyanine7 anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103224

Clone Number:

RA3-6B2

Staining for B220 in mouse samples with this antibody is very strong. Easy to separate B cells by flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymph node

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Single cell suspensions from mouse lymph nodes stained with this antibody show strong signal in B cells. Population is clearly separated from other cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

N/A

The Bottom Line

Great staining of B cells from mouse lymph nodes. There is some background, but not too much.

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