Great FOXP3 Antibody for Flow Cytometry

July 22, 2023

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

Invitrogen

Product Name:

FOXP3 Monoclonal Antibody

Catalog Number:

12-5773-82

Clone Number:

FJK-16s

Infiltration of FOXP3+ Regulatory T- cells into the brain during a mouse model of neuroinflammation is assessed by flow cytometry. Antibody for FOXP3 is first tested with cells from the spleen.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse spleen cells

Primary Incubation

1:200, 30 minutes on ice

Blocking Agent

TruStain FcX™ (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S flow cytometer (Beckman Coulter)

Results Summary

A population of FOXP+ CD4+ T-cells in the spleen was easily identified by flow cytometry. Regulatory T-cells in the brain of mice during neuroinflammation will be assessed with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Available conjugated to different fluorophores.

The Bad

Staining was not very bright.

The Bottom Line

This antibody worked very well to identify regulatory T-cells in mouse spleen cells by flow cytometry.

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