Excellent Ly6G Antibody From BD Biosciences

July 22, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BUV395 Rat Anti-Mouse Ly-6G

Catalog Number:

563978

Clone Number:

1A8

Info: Supplier Page 

Browse Similar Products: Anti-Ly6g Antibody Products

This antibody very specifically stains neutrophils in the mouse intestine. There is also little background, but still, it is a great addition to flow panels.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

The staining of Ly6G on neutrophils with this antibody is excellent. The dye is decently bright and the neutrophils can easily be identified if CD11b is included.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good staining.

The Bad

None.

The Bottom Line

Great staining to identify neutrophils. Definitely recommend.

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