Excellent MHC-II Antibody From BD Biosciences

July 22, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Horizon™ BV650 Rat Anti-Mouse I-A/I-E

Catalog Number:

563415

Clone Number:

M5/114.15.2

The staining is extremely bright. I'd recommend using much less of this antibody compared to others, especially when running flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestine

Primary Incubation

1:500 dilution for 15 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Excellent staining for mouse MHC-II. Antibody is very bright so decrease the concentration when using.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

N/A.

The Bottom Line

This antibody stains mouse MHC-II very well. Use a lower concentration when staining otherwise the signal will be too bright.

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