Great Antibody for Distinguishing B cells by Flow Cytometry From BioLegend

July 22, 2023

BlueskyReddit
Wayne State University

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD19 Antibody

Catalog Number:

115549

Clone Number:

6D5

Very good antibody for distinguishing B cells by flow cytometry. Some background is present, but the B cells are easily recognizable.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse intestine

Primary Incubation

1:200 dilution for 15 min

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Antibody works nicely to detect B cells by flow cytometry. Some background from myeloid compartment, but B cells can easily be distinguished as either SSCA low or B220+.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

Some background is present.

The Bottom Line

Great antibody for distinguishing B cells by flow cytometry. Recommended if color panel allows.

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