Thermo Fisher Scientific
GelCode Blue Stain Reagent
24592
Info: Supplier Page
I am interested in understanding the mechanisms of oncogenic WNT signal transduction. I use precise genome editing by CRISPR/Cas9 and ssODN to generate endogenous mutations in the components of the WNT pathway to understand their role in the WNT signal transduction. This method requires Cas9 protein and so in this experiment, I purified Cas9 protein from E. coli, and the purity was determined by staining the gel with the GelCode Blue reagent.
Staining of a 4-12% Bis-Tris Gel
Purified protein
Wash gel with water for 10 min. Repeat two more times before adding the reagent.
I get good staining if the gel is pre-washed with ultra pure water for three times. They typically stain for 1-3 hours followed by several washes of ultra pure water.
N/A
High quality protein staining reagent.
None.
Follow manufacturer's instructions to get superior quality result.