Excellent Staining for Mouse Blood Immune Cells From BioLegend

July 14, 2023

BlueskyReddit
Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ Anti-mouse CD45 Antibody

Catalog Number:

103149

Clone Number:

30-F11

Staining on immune cells from mouse blood is strong and specific. There is a little background and gives excellent separation between positive and negative populations by flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse blood

Primary Incubation

1:200 for 15 min at 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fortessa LSRII

Results Summary

Fluorescence signal is strong. There is excellent separation between positive and negative populations when run by flow cytometry, making the analysis easy and straight forward.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

N/A

The Bottom Line

If your color panel allows, definitely use this antibody. Great separation of populations by flow.

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