Excellent HuC/D Antibody From Abcam

July 14, 2023

Wayne State University

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-HuD + HuC Antibody

Catalog Number:

ab184267

Clone Number:

EPR19098

Excellent for immunofluorescence imaging of the mouse enteric nervous system. Very high specificity with little to no background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse intestine muscularis

Primary Incubation

1:500 overnight at 4°C

Blocking Agent

PBST + 5% donkey serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZEISS LSM880

Results Summary

Fluorescence signal from myenteric plexus is very strong. There is little background, allowing excellent visualization of the enteric neurons.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

Expensive.

The Bottom Line

This antibody works wonderfully for intrinsic enteric neurons.

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