Great FLAG Antibody From Sigma Aldrich

July 17, 2023

BlueskyReddit
Biophysics and Biochemistry
University of California, San Francisco
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Monoclonal ANTI-FLAG® M1 Antibody

Catalog Number:

F3040

Clone Number:

N/A

Info: Supplier Page 

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It is a great antibody used to detect expression levels of a protein that was FLAG-tagged for purification for cryoEM studies.

Experimental Design and Results Summary

Applications

Western blot

Sample

Expi293 cells (human)

Primary Incubation

5% BSA, 1:2000

Blocking Agent

3% milk

Secondary Incubation

3% milk

Tertiary Incubation

N/A

Detection

HRP

Results Summary

Very clean antibody and helped detect my tagged protein of interest very well and easily. We used it to test best level of expression for my protein for purification and cryoEM.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clean and easy to use.

The Bad

Nothing bad to say.

The Bottom Line

Worth the buy.

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