Good Antibody for Western Blot

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Claudin-5 Rabbit mAb

Catalog Number:

49564S

Clone Number:

E8F3D

BBB dysfunction in mice with respiratory infection was assessed by analyzing Claudin-5 expression via Western blot in whole brain lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse whole brain lysate

Primary Incubation

1:1000

Blocking Agent

PBS + 0.01% Triton-X100 + 5% BSA

Secondary Incubation

Anti-rabbit IgG HRP-conjugated 1:2000

Tertiary Incubation

SignalFire™ ECL Reagent

Detection

Licor Odyssey XF

Results Summary

Claudin-5 expression in whole brain homogenate from healthy and infected mice was measurable by Western blot with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal.

The Bad

None.

The Bottom Line

This antibody worked very well to analyze Claudin-5 expression by Western blot.

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