Good T cell Antibody for Immunofluorescence

July 16, 2023

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

Invitrogen

Product Name:

CD3 Monoclonal Antibody

Catalog Number:

14-0032-82

Clone Number:

17A2

T-cell infiltration into the brain in mice with respiratory viral infection was assessed by immunofluorescence microscopy with an antibody for CD3.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain tissue

Primary Incubation

1:500 in PBS + 0.01% Triton-X100, overnight at 4°C

Blocking Agent

PBS + 0.01% Triton-X100 + 10% BSA

Secondary Incubation

Goat anti-rat IgG AF647 1:500 in PBS+0.1% Triton-X100, 2hr at 25°C

Tertiary Incubation

N/A

Detection

Epifluorescence microscopy

Results Summary

CD3+ T-cells were detected by immunofluorescence microscopy in the perivascular regions of the brain of mice with respiratory viral infections.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Only a low concentration of antibody is needed for staining.

The Bad

Some background staining is apparent.

The Bottom Line

This antibody works well for staining histology samples for immunofluorescence imaging. However, it does have some background staining that can make analysis difficult.

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