Great Beta-Actin Antibody From Cell Signaling Technology

Results Summary

Cell lysate collected from syngeneic mouse models. The immunoblotting procedure is described below. Lyse cells by adding RIPA (with Proteinase and Phosphatase inhibitors) buffer. Heat 20µg of protein to 95–100°C for 5 min. Load 20µg of protein onto SDS-PAGE gel. Electrotransfer to PVDF membrane (Semi-dry, 20V, 1 and half hours). Incubate membrane in blocking buffer (1X TBST with 5% nonfat dry milk) for 1 hour at room temperature. Incubate membrane with diluted (1:1,000) primary antibody in 5% BSA, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Incubate membrane with the Rabbit-specific HRP-conjugated secondary antibody in blocking buffer with gentle agitation for 1 hour at room temperature.