Cuvettes for Medium-Large Quantity Cell Electroporation

June 28, 2023

BlueskyReddit
University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Lonza

Product Name:

P3 Primary Cell 4D X Kit L (24 RCT)

Catalog Number:

V4XP-3024

We equipped the OU lab with the Lonza 4D Nucleofector System for the electroporation of different cell types. The system requires dedicated consumables and we tested different vessel sizes. These cuvettes can host large amounts of cells (up to 10M) in a relatively small volume of 100 ul and reactive protein/RNA complexes or plasmid DNA. The kit is compatible with a large list of primary immune cells, both from humans and mice. We validated the kit for primary lymphocytes T (human/mouse) and lymphocyte B-derived cell lines.

Experimental Design and Results Summary

Application

Cell Electroporation

Starting Material

Primary Lymphocytes T and B cell line.

Tips

Not less that 3M cells per reaction or it looses efficiency.

Results Summary

Excellent results in matter of transfection efficiency and and cell viability. The small volume and the high concentration of the cells allow to save expensive reactives, such as Cas9 protein and RNAs. The large amount of efficiently electroplated cells per run is sufficient to perform complete experiment with several conditions.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, click and go - one kit is compatible with many cell types.

The Bad

Programs are fixed and consumables are of single use.

The Bottom Line

Excellent results for primary cells DNA or protein transfer with high viability. The large number of cells processed at once allows to reduce the number of electroporations required to fill all the conditions of the experiment. The fixed programs and kit grant easy manipulation and reproducibility.

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