Human Phospho-H2AX ELISA Works Well as I Expected

OHSU
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

RayBiotech

Product Name:

Human Phospho-H2AX (S139) ELISA

Catalog Number:

PEL-H2AX-S139-1

I analyzed EV (extracellular vesicle) samples.I detected Phospho-H2AX (S139) on my samples with high confidence.Positive control perfectly work as I expected.It would much better to provide 2 vials of positive control. Because once it resuspension with solvent, it could not keep it for next assay. I just run 2~3 samples at one time. Reduced amount of positive in 1 vial (50% less) and make 2 vials with 50% less materials in the vials.I would be very happy if provided positive control's protein concentrations. And it would be more confidence if the company provide more wash buffers.

Experimental Design and Results Summary

Application

ELISA

Starting Material

EV (extracellular vesicle)

Tips

Only 1 vial of positive control (can't keep it).

Results Summary

I analyzed EV (extracellular vesicle) samples. I detected Phospho-H2AX (S139) on my samples with high confidence. Positive control perfectly work as I expected. It would much better to provide 2 vials of positive control. Because once it resuspension with solvent, it could not keep it for next assay. I just run 2~3 samples at one time. Reduced amount of positive in 1 vial (50% less) and make 2 vials with 50% less materials in the vials. I would be very happy if provided positive control's protein concentrations. And it would be more confidence if the company provide more wash buffers.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Easy to run.

The Bad

Only 1 vial of positive control (can't keep it).

The Bottom Line

Highly recommended for EV work.

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