Cell Signaling Technology
Phospho-CREB (Ser133) (87G3) Rabbit mAb
9198
N/A
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Our research involves studying compartmentalized GPCR signaling and activity of downstream effectors, such as transcription factors.
Western blot
HeLa and HEK293 cell lysates
1:1000, overnight, 4°C on shaker
3% milk in TBS-Tween
1:10000, 1hr, RT on shaker
Chemiluminescent substrates, 2min RT
ECL then film/image dock
Helped us detect CREB activity via phosphorylation downstream of cAMP production at distinct membrane locations.
Decent detection.
Not that clean, there is some background noise.
Overall the product is okay, can detect phosphorylated CREB but not super clean.