Abcam
Mouse phospho-VASP (Ser157)
ab58555
N/A
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My research involves studying compartmentalized dopaminergic signaling. Here I am studying the activation of effectors downstream of receptor activity at distinct locations within a cell.
Western blot
HeLa and HEK293 cell lysates
1:1000, overnight, 4°C on shaker
3% milk in TBS-Tween
1:10000, 1hr, RT on shaker.
Chemiluminescent substrates, 2min RT
ECL then film/image dock
This antibody helped detect the activation (via phosphorylation) of VASP, an effector downstream of dopamine receptor signaling.
Clean, works well.
Expensive.
Works well but expensive, specific to phosphorylation site.