Secondary Antibody AF488 From Thermo Fisher With Great Signal

July 15, 2023

BlueskyReddit
MCB
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488

Catalog Number:

A48282

Clone Number:

N/A

We used this antibody as a secondary to detect GFP+ cells from tissue sections for immunofluorescence. It works well with very little antibody (1:1000 dilution). The green color on this image shows cytosolic GFP staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymph nodes

Primary Incubation

Anti-GFP antibody 1:2000, 1hr, RT

Blocking Agent

Blockaid

Secondary Incubation

Goat anti-rabbit AF488 (1:1000), 1hr, RT

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

We used this antibody as a secondary to detect GFP+ cells from tissue sections for immunofluorescence. It works well with very little antibody (1:1000 dilution). The green color on this image shows cytosolic GFP staining.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

None.

The Bottom Line

Antibody has great signal, highly recommend.

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