Good CD49b Antibody for Flow Cytometry

July 02, 2023

BlueskyReddit
MCB
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse CD49b APC/Cy7

Catalog Number:

108920

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD49b Antibodies

We use this antibody to identify natural killer cells from NOD mice by flow cytometry. Since NOD NK cells do not express NK1.1, this antibody is crucial for staining immune cells from NOD mice. We usually use this as a dump marker to gate out NK cells because we are interested in other myeloid cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200, 30min, 4°C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We use this antibody to identify natural killer cells from NOD mice by flow cytometry. Since NOD NK cells do not express NK1.1, this antibody is crucial for staining immune cells from NOD mice. We usually use this as a dump marker to gate out NK cells because we are interested in other myeloid cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

None.

The Bottom Line

This antibody has great signal, highly recommend.

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