Great Antibody With Good Staining

MCB
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse MHCII AF700

Catalog Number:

107621

Clone Number:

N/A

This antibody is very useful for identifying antigen-presenting cells with class II expression, from mouse lymphoid tissues. We use this regularly to find MHCII+ dendritic cells by flow cytometry as well as, to sort out dendritic cells from mouse lymph nodes for RNA sequencing.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:500, 30min, 4°C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody is very useful for identifying antigen presenting cells with class II expression from mouse lymphoid tissues. We use this regularly to find MHCII+ dendritic cells by flow cytometry as well as, to sort out dendritic cells from mouse lymph nodes for RNA sequencing. The staining is very good for DCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable and good signal.

The Bad

None.

The Bottom Line

Antibody has great signal, highly recommend.

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