Easy DAPI Staining for Microscopy

May 31, 2023

BlueskyReddit
Robert Bosch Gesellschaft für medizinische Forschung mbH
IKP/RBCT
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

ProLong Diamond Antifade Mountant

Catalog Number:

P36961

This ProLong mounting medium with DAPI is an easy-to-use mounting medium for fixed microscopy samples. I regularly use it for my fluorescence/confocal microscopy samples (fixed cells) which gives a nice nuclei staining which remained stable for several days and preserves the rest of the sample well for many weeks.

Experimental Design and Results Summary

Application

Fluorescence microscopy/Immunofluorescence staining.

Starting Material

Fixed cells (human cell lines) on coverslips

Tips

In some cases the DAPI staining remained only stable for a week or two. Samples should be imaged asap. 5 µl of medium is enough for a 12 mm coverslip.

Results Summary

After mounting samples, the mounting medium usually solidifies well over night. The staining is stable for at least a couple of days. However, background is possibly higher than for mounting media without DAPI.

DOI or PMID #

None

Image Gallery

Summary

The Good

Easy-to-use.

The Bad

Limited stability, high background.

The Bottom Line

For everyone who wants to have an easy-to-use DAPI staining combined with the mounting step I can recommend to try this product. However, the combination of these steps might have draw-backs in staining stability and background staining as mentioned above.

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