Easy to Optimize ERK Antibody

May 24, 2023

BlueskyReddit
IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p44/42 MAPK (Erk1/2)

Catalog Number:

4695

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. This antibody is used to detect the changes in the expression levels of ERK protein in samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine Immune Cells

Primary Incubation

1:1000, O/N, 4 degree

Blocking Agent

5% BSA TBST

Secondary Incubation

1:10000, 1hour, RT

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

The data showed the detection of 2 specific protein bands and there is no non-specific bands that were detected using this method.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to optimize and use.

The Bad

N/A

The Bottom Line

This antibody is highly sensitive and specific. It is easy to detect and does not require much effort to optimize. This antibody is recommended to those who wish to use western blot for the detection of ERK protein in their sample.

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