Great Signaling with Origenes' CD56 (NCAM) for IHC

Afyon Kocatepe University

Overall

Quality of Results

Ease-of-Optimization

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Company:

OriGene Technologies

Product Name:

CD56 (NCAM1) Mouse Monoclonal Antibody

Catalog Number:

TA506208S

Clone Number:

OTI1G4

Info:Supplier Page View Product Specs

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Great signaling for canine tissues. It is useful for differential diagnosis of canine neuroendocrine tumors.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Dog striated muscle tumor

Primary Incubation

1/200 dilution, diluent BSA %1, 2 hours at RT

Blocking Agent

%1 BSA

Secondary Incubation

Biotinylated anti-mouse IgG

Tertiary Incubation

Peroxidase conjugated streptavidin - AEC chromogen

Detection

ABC-peroxidase method

Results Summary

I use it in the differential diagnosis of some canine tumors. As you can see in the photo, it stains the skeletal muscle tumor positively, as expected, with a good dilution rate.

DOI or PMID #

N/A

Additional Notes

Gill's hemalum as a background staining.

Image Gallery

Summary

The Good

It binds specifically to expected tissues.

The Bad

Nope

The Bottom Line

Cheap and great signaling.

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