Good Iba1 Antibody for Staining Microglia

May 19, 2023

BlueskyReddit
OBGYN
University of Texas Medical Branch at Galveston
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Alexa Fluor® 568 Anti-Iba1 antibody

Catalog Number:

ab221003

Clone Number:

N/A

Info:Supplier Page View Product Specs

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We're doing a triculture of neurons astrocytes and microglia. Lights up microglia nicely in red color. Ab is conjugated so no need to add secondaries.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Cell culture

Primary Incubation

4 deg overnight

Blocking Agent

BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Lights up microglia nicely (red). Microglia can be distinguished from a triculture via morphology and Iba1 staining. Dilution at 1:100. Quick and easy to use.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and high quality, no secondary antibodies needed.

The Bad

N/A

The Bottom Line

Antibody has great signal, will recommend for microglial studies.

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